The Construction of Shuttle Plasmid Vector of Streptomyces and E. colt 链霉菌和大肠杆菌穿梭质粒载体的构建
Construction of a Recombinant Adenovirus Plasmid Vector Containing ESAT-6 Gene and CFP-10 Gene of Mycobacterium Tuberculosis 含结核分枝杆菌ESAT6和CFP10基因的重组腺病毒表达载体的构建
Experimental Study on siRNA Screening of Cathepsin K Gene and Construction of Its Plasmid Vector cathepsinK基因siRNA的筛选及其质粒载体的构建
Influence of Bifidobacterium plasmid polymerase on stability of plasmid vector 双歧杆菌质粒聚合酶基因对质粒载体稳定性的影响
Construction of Site-Directed Mutagenic Plasmid Vector of Porcine Myostatin Propeptide Gene and Preliminary Functional Analysis 猪肌肉生长抑素前肽基因定点诱变载体的构建和功能初步检测
At present, recombinant virus vector is one of the most hotspots in virus gene engineering, There are two vector type, the replaceable recombinant virus vector and the recombinant viral plasmid vector. 重组病毒载体又是当今病毒基因工程研究的热点之一,目前以动物病毒为基础设计的基因克隆及表达载体主要有取代型的重组病毒载体和重组的病毒-质粒载体。
Objective To investigate the transfection efficiency of plasmid vector coding enhanced green fluorescence protein ( pEGFP) in human umbilical vein endothelial cell ( HUVEC) by using ultrasound exposure and ultrasound contrast agent ( SonoVue). 目的探讨造影剂声诺维(SonoVue)联合超声辐照人脐血管内皮细胞(HUVEC)时增强型绿色荧光蛋白报告基因质粒(pEGFP)的瞬时转染效率。
ResuIts: The result of restriction fragment analysis tallied with the structural map of hTR plasmid vector. 结果:酶切鉴定的结果与hTR质粒图谱吻合。
Construction of New Plasmid Vector pPRS-1 of Cyanobacterium 蓝细菌新质粒载体pPRS-1的构建
Inhibitory effects of short hairpin RNA mediated by plasmid vector on cerb-B1 gene expression in human colon cancer cells 质粒介导的短发卡状RNA特异性抑制大肠癌cerb-B1基因的表达
The construction of the plasmid vector of human parathyroid hormone gene 人甲状旁腺激素基因表达载体的构建
Construction of a Ribozyme Transgenic Plasmid Vector and Its Inhibition to TMV Infection in Tobacco Protoplasts 核酶转基因载体的构建及在烟草原生质体中对TMV感染的抑制作用
Construction of the plasmid vector containing the antisense C gene of HBV 乙型肝炎病毒C区反义基因载体质粒的构建
It was therefore preparation for constructing an expression plasmid vector to express exogenous genes. 为构建其启动外源基因的质粒表达载体作准备。
Construction of the shuttle plasmid vector for fusion protein gene and expression in Hepatocellular Carcinoma cells 携带融合基因穿梭质粒的构建及在人肝癌细胞中的表达
The Research on the Construction of FasL Plasmid Vector and Its Anti-rejection Effect in Mice Heart Transplantation FasL质粒载体构建及在小鼠心脏移植中抗排斥反应的研究
Conclusion We have successfully constructed the recombinant PUC-hU_6-extra plasmid vector that can efficiently transcribe small RNA molecules directed by hU_6 snRNA promoter in the gastric carcinoma cells& SGC-7901. 结论成功构建了以hU6snRNA启动子驱动的、能在人胃低分化腺癌SGC-7901细胞内高效转录小分子RNA的表达质粒PUC-hU6-extra。
Study on construction of a plasmid vector carrying human hepatocyte growth factor gene and activity of its expression product 人肝细胞生长因子基因表达质粒的构建及其活性研究
Objective To construct a plasmid vector expressing the short hairpin RNA ( shRNA) targeting proliferation cell nuclear antigen ( PCNA), and to investigate its effect in vitro on the expression of PCNA, proliferation and apoptosis of human osteosarcoma cells. 目的构建增殖细胞核抗原(PCNA)短发夹状RNA表达载体,检测其对人骨肉瘤细胞PCNA表达、细胞增殖及细胞凋亡的影响。
AIM: To study the gene silencing effect of short hairpin plasmid vector on aquaporin-4 ( AQP-4) in astrocytes cultured in vitro. 目的:研究短发夹环质粒载体对体外培养的星形胶质细胞水通道蛋白4表达的基因沉默效果。
Hind ⅲ C fragment of replicative form ( RF) DNA of mink enteritis virus ( MEV) was cloned into plasmid vector pBR322, and which designated as pBM. 将貂肠炎病毒中间复制型DNA(MEVRFDNA)的HindⅢ酶切C片段克隆到pBR332中,形成重组质粒pBM。
Objective To construct mammal expression plasmid vector containing human BMP-2 for the study of gene therapy of bone defect disease. 目的构建人骨形态发生蛋白2(BMP-2)真核表达载体,鉴定并转染兔骨髓基质细胞,为转基因治疗骨缺失疾病提供实验基础。
Construction and Overexpression of The Plasmid Vector of 3 α-Hydroxysteroid Dehydrogenase From Comamonas testosteroni 睾酮假单胞菌3α-羟类固醇脱氢酶基因的质粒载体构建及表达
Construction of nos/ huifna chimeric gene and its fusion with Ti plasmid vector NOS/HuIFNa嵌合基因的构建及其与Ti质粒载体的拼接
Construction of the Retroviral Recombinant Plasmid Vector Encoding Thymidine Kinase Gene of the Herpes Simplex Virus and DNA Sequencing HSV-tk基因逆转录病毒重组体的构建与DNA序列分析
The gene sequences divided into three successively inserted into a plasmid vector, PUC19/ 18. 将其全基因序列分三段先后插入到PUC19/18质粒载体中。